My primers are designed to yield 1400 bp fragment. But on the gel after PCR, I am getting sharp band at 1000 bp and faint band at 1500 bp. What are the troubleshoot options that I can apply to get my band at appropriate size?
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 452 3 View
In Zn-Air Battery, how to plot Potential vs current density(mA/cm-2) in EC-Lab softare/BioLogic instrument. Please suggest. plot of the ref....
04 July 2024 3,807 2 View
and will i add phytochemical along with FUDR?
28 June 2024 6,254 0 View
As many of us know, there is often confusion regarding the alignment of dates, months, and seasons in our agricultural practices. To address this, I have prepared a table that aims to provide a...
27 June 2024 7,749 1 View
In Zn-Air Battery experiment, how to plot Potential vs current density(mA/cm-2) from discharge voltage curves. Please suggest.
23 June 2024 860 2 View
A masonry structure modelled all the wall using macro element and performing non linear time history analysis in dynamic explicit . I need to determine the random cracks where a give stress...
01 June 2024 1,349 1 View
Hi, I want to calculate the plastic equivalent strain for Mohr-Coulomb. I found a formula from Abaqus documentation. I have attached the photo of PEEQ calculation. But I want to figure out the...
28 May 2024 9,080 1 View
If the Fuzzy logic controller is not giving the desired results than what to do ? Further more...How to know how many membership functions and which type should be chosen?
20 May 2024 627 0 View
My student is isolating phages against ESKAPE pathogens, but she is getting a minute plaque. Can you please suggest how to get better results?
07 May 2024 9,554 2 View
I have added references using Mendeley reference manager, but it ruined the manuscript; it doesn't take ACS or other journals references properly except elsevier, and multiple duplicates are...
22 April 2024 6,559 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,666 3 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,597 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,113 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,056 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View