In soft tissue, why do lower kV x-rays produce more scatter and I guess have a higher RBE? I understand higher energy x-rays have more of a forward momentum, but I would think that the Compton process would be more damaging than the photoelectric.
I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?
03 April 2019 8,584 2 View
I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...
10 November 2017 5,622 3 View
What is the difference between measuring IFN-y positive PBMCs with and ELISpot and using FACS to measure the % of CD8+ antigen specific T-cells? What are the functional differences between the two...
08 September 2016 6,609 0 View
I'd like to know if it would be better to increase the number of cells plated or increase the peptide concentration? Which one would result in a better increase in spot formation.
03 April 2016 10,210 5 View
I'm isolating mouse PBMCs for an ELISpot assay. I work as quickly as possible but the cells do sit for 60-90 min while I'm counting cells and getting everything ready for the assay. The PBMC are...
02 March 2016 2,834 9 View
I'm growing 4T1 cells in mice. I'm implanting two contralateral tumors per mouse. I'm having a hard time getting the tumors to grow symmetrically. I understand they won't all be perfect, but I'm...
05 June 2015 8,163 7 View
I'm measuring BCL-2 by R&D's total humanBCL-2 Duo set ELISA. I'm treating cultured SK-OV-3 cells grown in low serum conditions. I'm wringing my hands as I can't show BCL-2 inhibition in the...
01 January 1970 8,642 2 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Require for Rietveld refinement in XRD.
08 August 2024 3,081 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
I will be doing an XRD on a sample which is powdered in natured and is a hydrogenated compound. I would like to know how we can understand the individual components and components by using the...
28 July 2024 4,548 2 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View