What is the role of IL-4 in generation of BM-DCs?

I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?

03 April 2019 8,428 2 View

Should I look at BCL-2 in whole cell or subcellular fraction?

I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...

10 November 2017 5,467 3 View

What are the differences between measuring T-cell response via ELISpot and FACS?

What is the difference between measuring IFN-y positive PBMCs with and ELISpot and using FACS to measure the % of CD8+ antigen specific T-cells? What are the functional differences between the two...

08 September 2016 6,478 0 View

What is the best way to increase signaling in an ELISpot assay?

I'd like to know if it would be better to increase the number of cells plated or increase the peptide concentration? Which one would result in a better increase in spot formation.

03 April 2016 10,037 5 View

How should I store PBMCs in serum free media that I've isolated for an ELISpot assay?

I'm isolating mouse PBMCs for an ELISpot assay. I work as quickly as possible but the cells do sit for 60-90 min while I'm counting cells and getting everything ready for the assay. The PBMC are...

02 March 2016 2,696 9 View

How can I establish good 4T1 tumors in-vivo?

I'm growing 4T1 cells in mice. I'm implanting two contralateral tumors per mouse. I'm having a hard time getting the tumors to grow symmetrically. I understand they won't all be perfect, but I'm...

05 June 2015 8,018 7 View

Protocl for measuring BCL-2 in SK-OV-3 cultured cells?

01 January 1970 8,477 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View