Derek Giuliana

8 Questions 6 Answers 0 Followers

Questions related from Derek Giuliana

What is the role of IL-4 in generation of BM-DCs?

I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?

04 April 2019 8,535 2 View

Should I look at BCL-2 in whole cell or subcellular fraction?

I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...

11 November 2017 5,579 3 View

What are the differences between measuring T-cell response via ELISpot and FACS?

What is the difference between measuring IFN-y positive PBMCs with and ELISpot and using FACS to measure the % of CD8+ antigen specific T-cells? What are the functional differences between the two...

09 September 2016 6,562 0 View

What is the best way to increase signaling in an ELISpot assay?

I'd like to know if it would be better to increase the number of cells plated or increase the peptide concentration? Which one would result in a better increase in spot formation.

04 April 2016 10,153 5 View

How should I store PBMCs in serum free media that I've isolated for an ELISpot assay?

I'm isolating mouse PBMCs for an ELISpot assay. I work as quickly as possible but the cells do sit for 60-90 min while I'm counting cells and getting everything ready for the assay. The PBMC are...

03 March 2016 2,790 9 View

Why is it that x-rays with lower mean effective energies produce more scatter?

In soft tissue, why do lower kV x-rays produce more scatter and I guess have a higher RBE? I understand higher energy x-rays have more of a forward momentum, but I would think that the Compton...

03 March 2016 6,950 0 View

How can I establish good 4T1 tumors in-vivo?

I'm growing 4T1 cells in mice. I'm implanting two contralateral tumors per mouse. I'm having a hard time getting the tumors to grow symmetrically. I understand they won't all be perfect, but I'm...

06 June 2015 8,124 7 View

Protocl for measuring BCL-2 in SK-OV-3 cultured cells?

I'm measuring BCL-2 by R&D's total humanBCL-2 Duo set ELISA. I'm treating cultured SK-OV-3 cells grown in low serum conditions. I'm wringing my hands as I can't show BCL-2 inhibition in the...

01 January 1970 8,599 2 View