Solvents with low-to-nil oxygen solubility?

I'm trying to find a solvent/liquid that will cause minimal oxidation to any surfaces it is applied to, and to suspended micro/nanoparticles. The solutes I wish to use may include CTAB, citric...

08 January 2019 542 2 View

Good source of diagrams of conductivity vs composition for alloys?

Hopefully this is self explanatory, but in case not, I'm looking for something like binary phase diagrams (or ternary), but the y-axis shows electrical or thermal conductivity rather than...

29 November 2018 2,798 7 View

Why is it that we can't quantify cDNA?

I know this is probably going to sound like a stupid question but why is it that you cannot quantify cDNA, as I've been told? Or is there? Just looking for some insight. Cheers, Mat

21 August 2017 6,434 1 View

Are there any self-report scales on work performance?

23 February 2017 3,936 5 View

What's the best mounting medium w/ DAPI?

Hi, I am doing in situ zymography to measure MMP activity in heart tissue sections. I am trying to decide between different mounting media w/ DAPI. 1. VectaShield Antifade w/ DAPI (H-1200) 2....

07 August 2016 6,979 7 View

Can FKBP proteins create a complex with a mammalian expressed protein?

I am currently trying to purify a protein called MMP-2. It has an approx. molecular weight of 72 kDa. For the past several weeks, I have been purifying this protein expressed from BL21-RIL E.coli...

11 December 2015 5,129 6 View

What happens if the Ni-NTA resin on my column runs dry?

Hi, I am doing an affinity chromatography protein purification for a protein of interest. I made the error of leaving my pump on too long and my buffer solution ran dry and made my column run dry...

09 October 2015 2,479 9 View

How can I tell if my protein is still bound to my resin?

07 August 2015 3,787 4 View

What's the best way to maximize protein binding to my column/resin in order to purify a protein with an Ni-NTA column?

I have done 2 purifications using a Ni-NTA column so far and each time we've noticed (based on my SDS-PAGE results) that lots of protein is collected in my flow-through (not binded to the...

06 July 2015 749 14 View

Will my pre-equilibrated Ni-NTA column be compromised if I run 20% ethanol through it? Will have to re-equilibrate my column with the appr. buffer?

I am doing a Ni-NTA chromatography purification of my protein. I noticed that air bubbles get caught within the column when I've poured my resin into it and I read somewhere that I can use 20%...

06 July 2015 7,618 9 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View