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Questions related from Barney .
I know this is probably going to sound like a stupid question but why is it that you cannot quantify cDNA, as I've been told? Or is there? Just looking for some insight. Cheers, Mat
22 August 2017 6,541 1 View
08 August 2017 4,326 1 View
Hi, I am doing in situ zymography to measure MMP activity in heart tissue sections. I am trying to decide between different mounting media w/ DAPI. 1. VectaShield Antifade w/ DAPI (H-1200) 2....
08 August 2016 7,094 7 View
I am currently trying to purify a protein called MMP-2. It has an approx. molecular weight of 72 kDa. For the past several weeks, I have been purifying this protein expressed from BL21-RIL E.coli...
12 December 2015 5,229 6 View
Hi, I am doing an affinity chromatography protein purification for a protein of interest. I made the error of leaving my pump on too long and my buffer solution ran dry and made my column run dry...
10 October 2015 3,107 9 View
05 October 2015 7,601 7 View
I am doing IMAC protein purification with a his-tagged protein but I have been having issues purifying my protein. First, the first few times I did it, I noticed that much of the protein was found...
08 August 2015 3,872 4 View
I have done 2 purifications using a Ni-NTA column so far and each time we've noticed (based on my SDS-PAGE results) that lots of protein is collected in my flow-through (not binded to the...
27 July 2015 852 14 View
07 July 2015 836 14 View
I am doing a Ni-NTA chromatography purification of my protein. I noticed that air bubbles get caught within the column when I've poured my resin into it and I read somewhere that I can use 20%...
07 July 2015 7,736 9 View