I'm new in the area and would like to know what makes IL-1 beta easier to detect in saliva as compared to the blood.
Dear Nur Basirah Ghazali!
Here is the basic of IL-1Beta detection in serum:
Evaluation of proinflammatory cytokine (TNF-alpha, IL-1beta, IL-6, IFN-gamma) concentrations in serum and cerebrospinal fluid of patients with neuroborreliosis].
[Article in Polish]
Kondrusik M1, Swierzbińska R, Pancewicz S, Zajkowska J, Grygorczuk S, Hermanowska-Szpakowicz T.
Author information
Abstract
BACKGROUND AND PURPOSE:
Neuroborreliosis is a tick transmitted disease which becomes an increasingly frequent diagnostic and therapeutic problem in physician practice. The purpose of this work was to evaluate the concentration of proinflammatory cytokines: IL-1beta, IL-6, TNF-alpha, IFN-gamma in serum and cerebrospinal fluid of patients with neuroborreliosis.
MATERIAL AND METHODS:
20 persons with diagnosed neuroborreliosis and 10 persons as a control group were examined in this study. The examination of serum and cerebrospinal fluid was performed twice, before and after 4-week therapy with antibiotics. The concentration of cytokines was measured by the ELISA method using kits of Bender Medical System and Quantikine RD Systems.
RESULTS:
The concentration of measured cytokines IL-1beta, IL-6, TNF-alpha and IFN-gamma in serum and cerebrospinal fluid was significantly higher before therapy. After 4-week therapy with antibiotics, the concentration of cytokines in cerebrospinal fluid decreased but was still higher than in the control group except for IL-1beta.
CONCLUSIONS:
The detection of proinflammatory cytokine concentration in serum and cerebrospinal fluid might be helpful as another parameter monitoring the inflammation course and therapy efficacy.
In addition, You have a fail, presenting detection of IL-1Beta in Saliva.
It is more complicated, but you have the detailed problematics and detection limits, what is most important!
Hope, you are satisfied, if not complete, ask again with the specific question!
Sincerely Yours!
Dr. Bratko Filipič
E-mail: [email protected]
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