Dear Scientists
I am trying to method validation of major phenolic compound from a medicinal plant. My standard curve recovery was an acceptable range (80 to 100%). But, when compound standards are spiking with extract after that recovery % is more than 200%. What types of solutions are needed to solve this problem? Please give me your valuable suggestions.
Thank you
Mr. Islam,
Reliable results can be obtained only my means of HPLC-MS. Consider the following discussion:
[1] [https://www.researchgate.net/post/How_do_I_Metabolomics_in_Thermo_Fisher_UHPLC-MS_MS].
Dear Ivanova
Thank you sharing your link but this is not relevant my quarry. My problem is spiking recovery is more than 200 % by HPLC-DAD analysis.
Dear Jinadasa
Thank you for your information. I will check and update you.
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