Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein?
Antibodies produced using as the immunogen a synthetic peptide or a recombinant protein will influence antibody specificity?
This is a very complex question, which may be good for several books. Since lacking validation is a persistent issue, this article might be a start: Article Ten Basic Rules of Antibody Validation
It depends on the protein (antigen), and the antigenicity which depends on the complexity n variability of the antigen
This is a very complex question, which may be good for several books. Since lacking validation is a persistent issue, this article might be a start: Article Ten Basic Rules of Antibody Validation
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