Ivana Zheng

5 Questions 7 Answers 0 Followers

Questions related from Ivana Zheng

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

09 December 2020 9,974 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

04 December 2020 405 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

25 November 2020 6,761 7 View

No target band in Co-IP assay?

I am trying to do CO-IP and I used two kits: Pierce™ Co-Immunoprecipitation Kit and Pierce™ Immunoprecipitation Kit. In both cases I don't have my target band (around 100 kDa) and I only have a 50...

20 October 2020 3,888 5 View

Why does mitotracker red stains the nucleus?

I'm working with NT2 cells and I use mitrotracker red (400 nM) to stain the mitochondria. I incubate it with cell medium for 30 min, fix with 4% PFA, permeabilize with PBST (0.2%-0.5% Triton...

12 August 2020 8,244 3 View