Hi,
I am cultivating my chlorella sorokiniana as heterotrophic. When the fermentation is ended the algaes are green. However, after some time (and not consistent time for each individual batch) the colour changes into brown. I've done pigments analysis which states that the chlorophyll a and b goes from around 25% for both down to 1% and the lutein goes from around 20% up to 50%. Overall, I wonder why my algaes are turning from green to brown? Also, why does the chlorophyll a and b goes down while the lutein goes up? Is it because of oxidation of chlorophyll a and b?
In regards, thanks.
Do you supply enough nutrients in the culture media?
Due to nutrient (nitrogen) deficiency, this may occur.
Kindly check https://www.hindawi.com/journals/tswj/2014/401265/
Also check https://www.nature.com/articles/s41598-020-59945-w
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