Why is dialysis important for FITC labelled chitosan NPs?

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Omar Gonzalez-Ortega

To eliminate free or hydrolized fitc.

Ekta Bhattacharya

Thank you so much. but wouldnt that be removed with multiple washing steps with suitable buffer?

Abdelhak Maghchiche

Dialyzing FITC-labeled chitosan nanoparticles in water removes unreacted FITC and purifies the solution, preventing background fluorescence. This step enhances the solubility and stability of the nanoparticles, making them more suitable for biological applications.