CTAB was established sometime ago as the best detergent to use during the extraction/isolation of highly polymerized DNA from plant material. This detergent simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins (enzymes). Thus, the DNA is not hydrolyzed during the isolation process and as long as vortexing or vigorous shaking are avoided highly polymerized (i.e., very high intact) genomic DNA is obtained.
Good luck.
It is a cationic surfactant. Its uses include providing a buffer solution for the extraction of DNA.We use this method for extracting genome sequencing quality (i.e. unsheared) DNA that can be used for large insert libraries.
Because of the broad distribution of negative charges in glycoproteins, these form broad, fuzzy bands in SDS-PAGE (Laemmli-electrophoresis). This can be avoided by using positively charged detergents like CTAB instead of the negatively charged SDS. Proteins can be blotted from CTAB-gels in analogy to western blots , and CTAB-PAGE can be used as second dimension after IEF.
Hi, maybe this will help you: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323937/
Cheers, Nadine
CTAB was established sometime ago as the best detergent to use during the extraction/isolation of highly polymerized DNA from plant material. This detergent simultaneously solubilizes the plant cell wall and lipid membranes of internal organelles and denatures proteins (enzymes). Thus, the DNA is not hydrolyzed during the isolation process and as long as vortexing or vigorous shaking are avoided highly polymerized (i.e., very high intact) genomic DNA is obtained.
Good luck.
AS Our colleagues Explained well apart from that I suggest you that CTAB provides great advantage of to obtain good quality of DNA because high conc. inert salts will help you to remove polysaccharides which are bind to DNA .
First, CTAB is a powerful cationic surfactant. It disrupts membranes and releases DNA. Second, plant tissues are rich in complex polysaccharides and secondary metabolites like polyphenols etc. These metabolites interfere and co-precipitate with DNA during the isolation procedure. CTAB along with some other chemical like Polyvinylpyrrolidone (PVP) is used to minimize the effect of these metabolites.
because Plant leaves of crop, tree and medicinal plants are rich in secondary
metabolites, polysaccharides and polyphenolics that are problematic during isolation of genomic DNA.
it shears the plant cell wall and membrane releasing the nucleic acid from the nucleus an as well, denatures the enzymes that might hydrolyse the nucleic acid such as DNases and RNases found in the cytoplasm of the cell
Methods are available that effectively remove polysaccharides and polyphenols from plant DNA preparations. The use of CTAB (cetyl trimethylammonium bromide), a cationic detergent, facilitates the separation of polysaccharides during purification while additives, such as polyvinylpyrrolidone, can aid in removing polyphenols. CTAB based extraction buffers are widely used when purifying DNA from plant tissues.
Do you think I can use Dicloromethane instead of Chloroform? Is there any advantage for chloroform over dicloromethane? Thanks!
I needed to look up CTAB, and Google landed me here at Researchgate. I found this answer most useful. This is in the context of my teaching work, students tend to Google for everything, and find everything and anything, and they are not at a stage of deciding what is most authenticated information. This is not to say that other answers are not reliable, but when discussing science, citing good publications is surely desirable.
I appreciate Nadine's answer as it is authentic, and also it avoids duplication, and answers without back-up of citation.
[4 years ago!] Nadine A. Gund Added an answer
Hi, maybe this will help you: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323937/ Cheers, Nadine
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology