Hi there,

Inclusion bodies formation is often described when overproducing one single protein so it is essentially formed from this overproduced material.

Inclusion bodies almost never occus when you are NOT overexpressing a protein. Only way to get mixed inclusion bodies is by overexpressing 2 proteins simultaneously.

Hi Priyankar, inclusion body formation is a stress response when cellular mechanisms can't properly fold overexpressed proteins.

http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2003.03785.x/full

Hello Priyankar Bhai,

First of All I am appreciating to you for nice question?

I am not very much sure but I am giving one hypothesis why only one protein is going into inclusion bodies. I am agreeing with Dominique Liger comments. The second question I am asking why they form very stable structures. I am  thinking that inclusion bodies form amyloid like structue because amyloid are very stable.  Due to over expression chances of stacking with same protein is more. So I think it could be a possible reason.