15 September 2023 25 2K Report

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More Igor V Minin's questions See All
Why don't I have a PCR product with proofread polymerase?

Dear Colleagues, I have the following problem: I’m trying to amplify a cassette with a resistance gene (about 2 kb) from the yeast genome, so that I can then insert it into a plasmid. With Taq...

03 July 2024 105 11 View

Staining of isolated mitochondria tecnique?

Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...

07 June 2024 5,909 0 View

How proving Riemann Hypothesis will fasten the development advanced self learning algorythms?

If Proven how Riemann hypothesis will help the development of AI and maybe help us create real advanced self-councious algorythms?

05 June 2024 9,787 3 View

Which relevant sources can be used to find reports on the scope of donations to political parties within the EU?

We are working on gathering raw data and information about the scope/share of private donations and public funding to pilitical parties that sometimes in EU is a part of responsibilities of...

16 April 2024 5,178 0 View

Is it possible to know the dwell time for a given isotope/mass from a raw LA-ICP-MS trace elements data file?

I have a dataset of trace elements LA-ICP-MS analyses with raw data, which consists of a set of a single file for each spot analysis. Each of these files has some columns (masses) and plenty of...

24 March 2024 898 6 View

Why are p-values of Durbin-Watson statistics different for dwtest and durbinWatsonTest in R?

I am analyzing some time-series data. I wrote a script in R and used two methods from two different packages in R to calculate the DW statistics and respective p-values. Surprisingly, for the...

14 March 2024 6,127 1 View

How to normalize expression data with SEM?

Greetings. Probably the question is not complex at all, but can't find an answer. If I have RT-qPCR data of gene expression in a sample with multiple analitycal replicates - to compare it to data...

13 March 2024 7,640 1 View

How can I add my papers with my name with different spelling?

In particular, I have few papers with spelling Agranovskii. Thank you

05 February 2024 3,764 1 View

How to generate causal effect estimates from causal forest in the grf R package for continuous treatments?

I am using the grf R package (https://grf-labs.github.io/grf/reference/causal_forest.html) to obtain causal effect estimates for a continuous treatment variable. The package description says:...

15 January 2024 1,990 0 View

What does a 4-dimensional Euclidean space look like from the point of view of a 3-dimensional observer?

If we keep in mind that R^{4}=R^{+}xS^{3}=R^{3}*RP^{1} where * means a direct product with a singularity at the zero point of a 3-dimensional Euclidean space in which the projective line is...

30 December 2023 9,110 1 View

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How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Do you agree that at some point in our lives each of us will be a person with disabilities?

There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...

17 July 2024 3,798 13 View

Which mesostigmatan mites from black soldier fly farm?

I have aquired some mites which have been isolated from feeding trays at a black soldier fly farm. These mesostigmatans are not my primary field of interest, although they are very beautiful. Can...

13 July 2024 10,036 5 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Any Green Solvents to dissolve PET near room temperature?

I need a PET + Solvent mixture to feed it to a fixed bed reactor for steam reforming.

30 June 2024 2,122 2 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

Where can I find my citations for my published papers?

1- M.M.M. Ahmed, F.M. El Hag, F.S. Wahab, S.F. Salih (2001). Feeding strategies during dry summer for lactating desert goats in a rainfed area under tropical conditions. Small Ruminant Research 39...

18 June 2024 552 5 View

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