I'm working with transformation of plasmid into Agrobacterium GV3101(Cells and protocols from Intact Genomics).

This strain has pMP90 as helper plasmid and it has a gentamicin resistance gene as described in Intact Genomics protocol introduction part.

In some protocols, including Intact Genomics's protocol, they use Rifampicin and Kanamycin for selection after transformation, but not Gentamicin.

I'm wondering that if I put Kanamycin and Rifampicin, helper plasmid pMP90 goes disappear.

Does anyone has a information about that?