Ooi Yoshito

2 Questions 6 Answers 0 Followers

Questions related from Ooi Yoshito

Why I don't have to do selection for Helper plasmid with Rhizobium radiobacter(Agrobacterium) SV3101 transformation?

I'm working with transformation of plasmid into Agrobacterium GV3101(Cells and protocols from Intact Genomics). This strain has pMP90 as helper plasmid and it has a gentamicin resistance gene as...

27 November 2024 485 0 View

How can I increase DNA concentration after gel purification for long length DNA fragment?

I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...

02 July 2024 5,021 9 View