Why I am not getting plaques of PEG precipitated virus?

09 September 2018 0 10K Report

I have been using PEG precipitation method for virus concentration. The virus I am using is fluorescent-tagged. After PEG precipitation the pellet is dissolved in appropriate buffer, which shows good florescence reading using fluorescent micro plate reader.

But when I am performing plaque assay to measure the pfu/mL of the virus, there are no plaques. What could be the reason ?

I am using PEG 8000 and 0.5 M NaCl solution for precipitation.

Please help!

Vedita

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Science method

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