Vedita Singh

3 Questions 16 Answers 0 Followers

Questions related from Vedita Singh

Why I am not getting plaques of PEG precipitated virus?

I have been using PEG precipitation method for virus concentration. The virus I am using is fluorescent-tagged. After PEG precipitation the pellet is dissolved in appropriate buffer, which shows...

09 September 2018 9,633 0 View

Which one is more efficient for cell disruption and protein purification , a constant cell disruptor system or a cell sonicator?

Here the cells used are E.coli DH5alpha.

08 August 2016 8,637 10 View

Dear all, I need suggestions regarding Splicing via Overlap extension PCR. Trying to join two 4kb Overlap fragments using HerculaseII?

I have amplified two fragments 4.0 kb each with overlapping segment. I am trying to join them using HerculaseII enzyme. The procedure I am following is this: PCR 1 (overlap PCR) for 10 to 15...

01 January 1970 177 10 View