In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it OK??

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it ok??

25 January 2021 5,336 10 View

Are two text files obtained separately from qiime 2 can be used together to make diversity graphs?

I have two sets of 16s microbiome sequencing data. The animal model, facility, diet vendor are the same in both sets. The only change is in the treatment. For analysis, can I use both the...

29 December 2020 6,976 3 View

How to estimate water volume using remote sensing?

I want to estimate water volume from different water surfaces. I have used Landsat data before. Is there any method to extract water volume from NDWI or other Landsat bands? If not then, what are...

21 October 2020 4,775 11 View

Any data available on traffic distribution for underwater wireless communication/sensor networks?

I am looking for any source of information that describes how often an underwater wireless communication network is utilised on a certain day. For example, if you consider an underwater wireless...

07 October 2020 4,340 6 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

Name of some mastermixes which contains UNG enzyme?

Hello, We are planning to work with HBV viral load. I have read several articles on HBV viral load quantification and came to know that they have used such mastermix that contains UNG enzyme to...

16 September 2020 7,911 2 View

Could you please recommend any good tutorials for natural ventilation in ansys with ref./pdf?

I am looking for a good tutorial for natural ventilation of a small room in Ansys fluent with ref/pdf for a detailed study.

22 June 2020 4,992 4 View

How to prepare 2x one step RT PCR mastermix?

Hello, I am working on developing a real time RT PCR based kit. For that, I need to prepare 2x one step RT PCR mastermix. I am using PCR Reagents and Verso cDNA synthesis kit from thermo...

03 May 2020 8,796 3 View

What kind of microstructure of Low Carbon Steel favors the maximum yield stress (>400 MPa)?

Micro-structural effect to Yield stress and yield ratio of low carbon steel.

13 March 2020 4,018 2 View

Why discontinuous yielding occurs?

The relation of discontinuous yielding with micro-structure of low plain Carbon steel

13 March 2020 527 4 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View