Is it possible to do Reitveld Refinement on single crystal XRD data?

I tried to do single-crystal Rietveld Refinement on my single-crystal XRD data using the option "refinement on single-crystal data/ integrated intensity data" in Fullprof but it is giving error...

30 January 2021 7,813 3 View

What will be the impact on Antenna performance to shift the resonant frequency by RLC Matching network?

There is always a possibility to shift the resonance frequency by adding a RLC matching network. I wonder what will be the consequence on antenna performance (axial ratio, gain)? If there is no...

13 September 2020 5,187 3 View

Axial ratio shows different result at different ortogonal setup. What could be the reason ?

17 June 2020 5,332 3 View

Firm survival and mortality: How to relate both?

I have two data sets 1. Age of all the surviving firms at any given point of time. The age distribution is an exponential function 2. The age of all the firms which died at a given point of time (...

01 February 2020 3,542 1 View

Why Antenna shows negative gain even though it has very good matching?

I am aware of the fact that electrically small antennas are poor radiators with very small gain and low efficiency. One main cause of this is poor matching. An extremly electrically small antenna...

19 January 2020 5,285 9 View

What is the impact of image current from ground plane on patch antenna? I wonder how it radiates if ground plane is very near to radiator?

As we know, patch antenna is combination of two magnetic dipole. If we place a perfect electrical conductor near a dipole, antenna performance will suffer significantly. Then how a patch antenna...

27 November 2019 7,597 5 View

I am getting two peaks at 702 and 705 eV for Fe 2p scan of a composite made from Fe-salen.how it can be explained?

the pure Fe-salen spectra is in conformity with literature,but the composite is showing two extra peaks at 702 and 705 eV. Can anybody explain about why the peaks are coming.

23 July 2019 5,710 3 View

What will be the cororidinate(theta and phi) to evaluate the axial ratio?

I have designed a circular polarized antenna. But , I am confused with cor-ordinate system. In some angel it shows less than 2 dB axial ratio and some other point it shows more than 10 dB like a...

23 May 2019 2,175 7 View

Why there is a big difference in between realize gain and gain in my design?

I have designed a multiport antenna. Where as, I have 2 dB Gain but realize gain is -6 dB. Point to be noted, S11 is really good. I have -25 dB return loss at my desired frequency.

22 November 2018 6,165 17 View

Bands in western blot are behaving like GHOST!!!!!!!!!!!!!!!!!?

I am doing western blot and after development I can see that one/ two lanes have no band. Perhaps, next day I am doing same gel run with same sample with no change in protocol, this time another...

25 September 2018 8,325 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View