Why HepG2 cell line is used for apoptotic researches?

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Reza Hadavi

as far as I know , Jurkat cell line used as a model for Apoptosis research . because this line has maximum Proteasome activity .

good luck

Go J Yoshida

Why not firstly treat with positive control (H2O2, Staurosporine (ab120056))?

And then check with AnnexinV/PI with FACS or perform WB to detect cleavaged PARP (89kDa) or active caspase 3 (15kDa).

http://www.abcam.co.jp/staurosporine-ab120056.html

Heshu Sulaiman Rahman

HepG2 cell line is very easy and fast to grow, low contamination can be seen, and available in almost all cancer labs. No need for special amino acid or supplement.