Dear Hani... being too small in size His tag is very poorly immunogenic.... means you can continue your immunogenicity testing with the protein........ no need to remove the His tag...... it will be well accepted for publication and no objection will be there because of His tag... You can also go through my publications in my profile.... In all the publications, the immunogenicity testing was done with His tagged purified proteins............ Best Wishes..........

thank you Dr.piyush Paliwal