Many protein refolding protocols describe the use of glutathione oxidized and reduced in the protein refolding step after solublization of over-expressed protein in the form of inclusion bodies.
What is the mechanism for this reaction?
I have sequence for Factor Xa site in my expression vector, which will get translated to Ile-Glu-Gly-Arg after protein expression. What is the purpose of this site?
02 March 2019 5,890 1 View
How can we find the function of a protein in the mammalian cells using molecular biology techniques?
10 November 2018 3,404 0 View
Nowadays, ageing research include various aspects of ageing like obesity, diabetes, neurodegeneration, cancer, kidney dysfunction, etc. Which is the recent best research paper related to above...
31 December 2017 6,860 5 View
What are the best conditions to improve transformation efficiency of XL1 Blue competent cells? Please suggest any other conditions that you use to troubleshoot transformation efficiency in XL1...
09 October 2016 7,214 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
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05 August 2024 4,407 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...
30 July 2024 7,062 1 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco
29 July 2024 2,163 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View