Many protein refolding protocols describe the use of glutathione oxidized and reduced in the protein refolding step after solublization of over-expressed protein in the form of inclusion bodies.
What is the mechanism for this reaction?
I have sequence for Factor Xa site in my expression vector, which will get translated to Ile-Glu-Gly-Arg after protein expression. What is the purpose of this site?
02 March 2019 5,733 1 View
How can we find the function of a protein in the mammalian cells using molecular biology techniques?
10 November 2018 3,213 0 View
Nowadays, ageing research include various aspects of ageing like obesity, diabetes, neurodegeneration, cancer, kidney dysfunction, etc. Which is the recent best research paper related to above...
31 December 2017 6,687 5 View
What are the best conditions to improve transformation efficiency of XL1 Blue competent cells? Please suggest any other conditions that you use to troubleshoot transformation efficiency in XL1...
09 October 2016 7,061 4 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?
02 March 2021 1,493 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...
02 March 2021 8,481 3 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View