Vikas Ranvir

5 Questions 11 Answers 0 Followers

Questions related from Vikas Ranvir

What is the use of Factor Xa Protease ?

I have sequence for Factor Xa site in my expression vector, which will get translated to Ile-Glu-Gly-Arg after protein expression. What is the purpose of this site?

03 March 2019 5,815 1 View

How can we find the function of a unknown protein in mammalian cells?

How can we find the function of a protein in the mammalian cells using molecular biology techniques?

11 November 2018 3,328 0 View

Why Glutathione-redox reaction is necessary for in-vitro protein refolding?

Many protein refolding protocols describe the use of glutathione oxidized and reduced in the protein refolding step after solublization of over-expressed protein in the form of inclusion...

06 June 2018 4,408 0 View

Which is the most important research paper that revolutionized the field of ageing research?

Nowadays, ageing research include various aspects of ageing like obesity, diabetes, neurodegeneration, cancer, kidney dysfunction, etc. Which is the recent best research paper related to above...

01 January 2018 6,795 5 View

Would heat shock at higher temperatures (45-47 degree C) improve transformation efficiency of XL1 Blue competent cells?

What are the best conditions to improve transformation efficiency of XL1 Blue competent cells? Please suggest any other conditions that you use to troubleshoot transformation efficiency in XL1...

10 October 2016 7,165 4 View