The reaction catalyzed by AlkB couples the oxidative decarboxylation of alpha-keto-glutaric acid to the hydroxylation of the methyl group. The methyl group then spontaneously decomposes to formaldehyde, restoring the original base such as 3- methyl cytosine to cytosine by AlkB,ABH2/3 and 2OG,Fe(II), O2.
But in some cases like conversion of 5-hydroxymethyl cytosine from 5-methyl cytosine by TET1 and 2OG,Fe(II), O2 OR 5-methoxycarbonyluridinie (mcm5U) hydroxylation by ALKBH8 and 2OG,Fe(II), O2 to form mchm5U in tRNA. They have the OH- group on the side chain which doesnt decomposes to formaldehyde (HCHO). How this reaction has happen? How methyl group decomposes in these two reactions? How these products 5-HMC and mchm5u are stable?
You can not expect that all the enzymes from a unique family catalyzes the same reactions.In many case you can see even a unique enzyme catalyzes two different reaction.Functionality of an enzyme depends on many factors.Presence of essential cofactor has a great effect on that.If you investigate more about the place that those reactions which you said happens in the cell and their conditions.If you search the following link for the enzyme you mentioned you can find useful information
http://www.brenda-enzymes.org/
Best Regards
Sepideh
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