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I have tried to purify His tag human protein (40kDa) from the Ni -NTA Beads slurry(Qiagen) from 1 ltr culture through Batch purification. I have transformed the plasmid into BL21(DE3) cells and...
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The reaction catalyzed by AlkB couples the oxidative decarboxylation of alpha-keto-glutaric acid to the hydroxylation of the methyl group. The methyl group then spontaneously decomposes to...
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