How far complementarity exists between solution NMR data and X-ray diffraction studies of macromolecular structures in Biology?

There should be understandable correlation between structural biology and physiological function, e.g.,...

03 April 2016 4,400 3 View

Is not an article published by making payment an advertisement?

Most online journals ask for payment for publishing research/review articles, under the pretext that no one is making money in the process. Is it really so.

02 March 2016 8,539 5 View

Do gram positive microbes bleb out membrane vesicles?

Gram negative microbes signal their environment via outer membrane vesicles http://en.wikipedia.org/wiki/Bacterial_outer_membrane_vesicles

05 June 2014 889 0 View

Does the term 'ultrastructure' include electron microscopy alone?

Is ultrastructure, seeing smaller than light microscopy http://pediaview.com/openpedia/Ultrastructure ?

04 May 2014 9,418 2 View

Should scientist be first a good citizen?

Should scientists feel social responsibility in choosing research projects.

04 May 2014 2,095 2 View

Why are viral infections, including the common cold, difficult to treat?

Antibiotics are given to safe guard against secondary bacterial infections due to reduced immunity during viral infections.

03 April 2014 5,221 5 View

What is the best time, pre- or post-meal, to take a dose of probiotics?

How to get the best health results from probiotics.

02 March 2014 5,540 2 View

Are multi-authored research papers cited more than single-author ones?

Is there any doubt or bais against citing single-authors research papers?

01 February 2014 3,944 2 View

What is a 'good' question?

Can a question be called 'good' if it is difficult to answer, or else, it generates interesting discussion?

01 February 2014 5,425 13 View

Does theoretical research have a much value in biosciences?

Most mark in bioscience research has been made by experimental and instrument-based research. Was DNA double helix breakthrough more theoretical?

01 February 2014 5,789 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View