Dear Researchers,

I am currently trying to standardize a cost effective protocol for DNA extraction from archived slides and FFPE tissues. After so many trial and error, I finally am getting DNA which I'm quantifying with a Nanodrop. The yield so far ranged from 10 to 450ng/ µL with purity ratio ranging from 1.65 to 1.97. I was pretty satisfied until I ran an agarose gel!! There is no trace of any DNA with whatever percentage of gel I use!! I know the nanodrop readings could be just because of degraded nucleotides but why can't I see any DNA bands?

Please share your thoughts.

Thanks!