20 February 2024 1 10K Report

Hi! I have a question regarding the use of EPF as a biomarker for pregnancy in cattle.

Why is Early Pregnancy Factor (EPF) not widely used as a biomarker for pregnancy detection in cattle, especially considering its immediate expression after embryo attachment to the uterus and its potential for rapid pregnancy detection tests using lateral flow immunochromatography?

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What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 646 3 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Help to isolate Ralstonia from plant tissue?

I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...

02 July 2024 9,806 3 View

How to design an online training, learning platform ?

when designing an e-learning platform what model and programming language do you select?

29 June 2024 7,502 4 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

I'm having trouble extracting total RNA from a culture of macrophages derived from monocytes infected with Leishmania infantum. Can someone help me?

I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...

12 May 2024 1,044 4 View

How can I set the distance between peptide and bilayer using Packmol?

I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...

29 April 2024 8,231 1 View

What requirements (or qualities) are most important to be a good university professor?

What requirements (or qualities) are most important to be a good university professor?

22 April 2024 3,009 5 View

How to calculate the solute concentration in molecular dynamics simulation?

I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...

16 April 2024 2,051 2 View

What are the factors contributing to the phenomenon of plant roots exhibiting a purple coloration?

In my doctoral research, i'm investigating the intersection of biotechnology and maize seed (Zea mays) productivity. An intriguing observation I have made pertains to the coloration of maize seed...

09 April 2024 1,726 6 View

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How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

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07 August 2024 1,027 0 View

Does exposure to toxins during pregnancy increase early puberty risk?

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How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

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28 July 2024 8,169 0 View

Will the catalytic effect of retained austenite happens in steels including RA?

The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...

27 July 2024 8,507 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

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Como e a rotina na educaçao infantil ?

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Best method for extracting RNA from morulae?

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21 July 2024 4,398 1 View

How can I calculate spin texture using Quantum Espresso for non-colinear case ?

I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??

19 July 2024 8,570 1 View

Why not cow dung manure used in lie for artificial ?

Cow dung manure is more powerful than artificial fertilizer.

19 July 2024 7,392 7 View

Is cow dung can be in used for repeated use ?

Is this organic cow dung manure now important than artificial fertilizer.if good then why ?

19 July 2024 9,050 2 View

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