Why does total mTOR protein expression decrease after long term exposure (around 12 hrs) to Rapamycin. Also, is there any reference? Thank you.

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Divaker Choubey

A prolonged treatment of cells with rapamycin, an inhibitor of mTOR, inhibits protein synthesis. Thus, depending upon the half-life of a protein, it is predicted to decrease the levels of proteins in cells. In certain cell types, the mTOR protein half-life is short (1.5 - 3 h). This could account for an observed decrease in the levels of mTOR following rapamycin treatment of ovarian cancer cells.

Abhilash Deo

Thank you Sir.