Dasilva Nuswantari

6 Questions 11 Answers 0 Followers

Questions related from Dasilva Nuswantari

Why does the SH-SY5Y cell line can undergo an automatisation of differentiating in a regular growth media after being cryopreserved?

I have cryopreserved the undifferentiated condition of SH-SY5Y cell line in the early passage in a cyomedia formulated with 90% FBS and 10% DMSO (sterile-filtered). Some months later, I have...

26 March 2024 1,193 2 View

What is the normal doubling time of SHSY5Y cell line?

I never handle the SHSY5Y cell line before, so it is a new thing for me, as far as I observed for these past five days, their growth is really slow. Is it normal? I use the complete media of...

03 August 2023 8,643 6 View

Is there any possibility for cell lines to recovered from its cryogenic phase, if it was found in a complete thawing condition already?

Hello, anyone. I have a technical questions related with cell lines recovery after it was found in a complete thawing condition, so, long story short, I have a technical accident where the liq N2...

20 May 2022 3,953 3 View

Can anyone give me a suggestion of how to make an antibiotic kill curve experiment in wild type cell lines?

I'm conducting a Puromycin dihydrochloride antibiotic selection in CHO cells, according to several protocols, the medium is replaced with fresh medium containing Puromycin every 48 hrs, in order...

19 August 2021 5,902 4 View

Can anyone help me identify a cell culture contamination?

I'm working with MDCK cells, but unfortunately there's a contamination appear on my cell culture with the some characteristics as follow; 1) the colour of the medium is clear yellow, not turbid;...

08 July 2021 9,512 7 View

Does anyone have a recommendation of using Serum Free Media that is suitable for adherent CHO and HEK293T culture for vaccine development?

This is my first time I will be using a Serum Free Media in my cell culture experiment due to vaccine development. I tried to ask to the commercial supplier regarding this, but mostly said that...

02 June 2021 4,219 3 View