To detect hydroxyl radicals, DMPO was used to obtain the following ESR spectrum, but small peaks between large peaks could not be seen as in other spectrums.
Can you tell me what's the problem?
Dear Chae-Lin Kim These small peaks are attribute to as stated hereArticle Silica Nanoparticle-Generated ROS as a Predictor of Cellular...
“The peaks in between the four signals corresponding to the DMPO spin adduct have been assigned to aminoxyl (IUPAC recommended name for [R2N–O ̇] [R2N ̇+–O−]) radical.”Pretty much the same as the info underneath the Figure 6 stated “An unidentified nitroxide metabolite (open circles) was also detected in the effluent.” https://www.sciencedirect.com/science/article/abs/pii/S0167730608604500
Best regards.
A similar ESR spectrum (with six lines) correspond to the adduct DMPO-oxide radical anion (the conjugate base of hydroxyl radical), which is obtained at very high pH (>12). Please see Redox Report (1997), 3, 303.
You might check your resolution by changing your modulation amplitude and use a power plot to find the best settings. Details on these parameters are missing in your questions.
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