Why does the presence of different contamination change the pH of media in different ways?
Different type of contamination like fungal make different types of metabolite which mostly acidic of slightly acidic. but if they can't tolerate that concentration of metabolite cells are going to dye due to cell lysis, thats why pH rises.
As we know so far, around 25 proteins and peptides are known to involve in amyloid disease. If we assume that the fibrillation is an intrinsic feature of all polypeptides, and that we have tens...
31 December 2018 3,241 3 View
In an assay if we find that the best pH for a protein which will be injected to the patients, is around 3, it is ok that during formulation, we use an acidic buffer.In similar situation if we...
04 May 2018 2,731 2 View
I want to have a cell line with high content of a-synuclein. I have looked at many reports which in them two different systems were used to over-express of a-synuclein. Does any one know which...
06 July 2015 3,377 6 View
Is it Ok to Analysis of the significantly / meaningfully of an inhibitory effect with anova-test or t-student?We work on a compound which can decrease the cytotoxicity effect of another compound...
11 December 2014 9,856 3 View
We are working with a protein to induce fibrillation forms. Our FTIR (deep peak at 1624 qnd 1695) results and AFM images (twisted long fiberils) confirmed fibril formation. In spite of these...
08 September 2014 2,650 10 View
When I look at the SDS-page pattern of alpha synuclein in some studies its band appears higher (around 17 KD) than the real place of its molecular weight (14.4 KD). However, our purified protein...
06 July 2014 9,222 3 View
In many proteins such as lysozyme and alpha-synuclein, only a limited part of protein participate in cross-beta sheet and fibril formation. In alpha synuclein from 140 residues only about 60...
01 February 2014 1,440 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View