Why just some proteins and peptids cause amyloid diseases?

As we know so far, around 25 proteins and peptides are known to involve in amyloid disease. If we assume that the fibrillation is an intrinsic feature of all polypeptides, and that we have tens...

31 December 2018 3,146 3 View

If a optimum pH for a pharamaocitical protein is so acidic or so basic, in its formulation, we can used aicdic or basic solutions?

In an assay if we find that the best pH for a protein which will be injected to the patients, is around 3, it is ok that during formulation, we use an acidic buffer.In similar situation if we...

04 May 2018 2,656 2 View

Which system is better for overexpresion of alpha-synuclein in a dopaminergic cell line such as SH-SY5Y : adenoviral vector or lentiviral vector?

06 July 2015 3,288 6 View

Is it acceptable to analyze the significance of an inhibitory effect with anova-test or t-student?

Is it Ok to Analysis of the significantly / meaningfully of an inhibitory effect with anova-test or t-student?We work on a compound which can decrease the cytotoxicity effect of another compound...

11 December 2014 9,777 3 View

Why are FTIR and AFM results inconsistent with Far-UV CD ones for the fibril form of a protein?

We are working with a protein to induce fibrillation forms. Our FTIR (deep peak at 1624 qnd 1695) results and AFM images (twisted long fiberils) confirmed fibril formation. In spite of these...

08 September 2014 2,563 10 View

Does anyone purify recombinant alpha synuclein?

06 July 2014 9,137 3 View

Why does the presence of mold and yeast cause pH elevation but bacteria cause opposite effect on cell culture?

Why does the presence of different contamination change the pH of media in different ways?

04 May 2014 3,104 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View