Why does the error says GDC server down while I try to download data from the GDC portal through bioconductor tool?

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How feasibile is it to simulate an ODE metabolic model with 38 variables?

I have been searching literature to proceed with a metabolic network which include the detailing of a process including around 38 variables and 25 unknown parameters. How feasible is it to make a...

31 December 2017 1,475 2 View

How good is to predict the structural properties of a protein across different organism based on its available sequence information?

It is assumed that proteins having a sequence similarity of ~35% tend to fold in a similar pattern. If so, can this be used to predict the binding properties of the two proteins based on their...

01 February 2017 5,395 0 View

What is the significance of finding a solution in the null space?

When a "m x n" matrix A is Linearly transformed such that A.x=0, then the solution is expected to be present in the null space. What does this means? And is it easier to find a solution in the...

08 September 2016 2,669 0 View

Is there an alternate for Opti-MEM for lipofectamine transfection? Can I use MEM with low supplemented FBS?

Opti-MEM is a modification of MEM only. Why is a low FBS required during transfetion?

05 June 2016 4,676 11 View

Can anyone provide me with a proper transfection protocol into HEK293 cells with Lipofectamine 2000?

I have ordered lipofectamine 2000 from Thermo Fishers Scientific. The product has been provided with a protocol, but does not clearly reports the incubation times. I have tried transfection as per...

05 June 2016 6,585 8 View

Is there a way to check whether Ligation has worked properly before doing transformation?

I have tried the ligation procedure quite many times, but have failed to get colonies after transformation. I get no colonies after transformation. Also I have tested the competence of competent...

01 February 2016 4,567 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View