Why does the error says GDC server down while I try to download data from the GDC portal through bioconductor tool?

I am trying to download DNA Methylation Data from the TCGA Bioconductor tool. But whenever I give a query I encounter this error: "Error in value[[3L]](cond) : GDC server down, try to use this...

04 May 2018 8,856 2 View

How feasibile is it to simulate an ODE metabolic model with 38 variables?

I have been searching literature to proceed with a metabolic network which include the detailing of a process including around 38 variables and 25 unknown parameters. How feasible is it to make a...

31 December 2017 1,475 2 View

How good is to predict the structural properties of a protein across different organism based on its available sequence information?

It is assumed that proteins having a sequence similarity of ~35% tend to fold in a similar pattern. If so, can this be used to predict the binding properties of the two proteins based on their...

01 February 2017 5,395 0 View

Is there an alternate for Opti-MEM for lipofectamine transfection? Can I use MEM with low supplemented FBS?

Opti-MEM is a modification of MEM only. Why is a low FBS required during transfetion?

05 June 2016 4,676 11 View

Can anyone provide me with a proper transfection protocol into HEK293 cells with Lipofectamine 2000?

I have ordered lipofectamine 2000 from Thermo Fishers Scientific. The product has been provided with a protocol, but does not clearly reports the incubation times. I have tried transfection as per...

05 June 2016 6,585 8 View

Is there a way to check whether Ligation has worked properly before doing transformation?

I have tried the ligation procedure quite many times, but have failed to get colonies after transformation. I get no colonies after transformation. Also I have tested the competence of competent...

01 February 2016 4,567 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View