When a "m x n" matrix A is Linearly transformed such that A.x=0, then the solution is expected to be present in the null space. What does this means? And is it easier to find a solution in the null space than in the column space? if yes, why?
I am trying to download DNA Methylation Data from the TCGA Bioconductor tool. But whenever I give a query I encounter this error: "Error in value[[3L]](cond) : GDC server down, try to use this...
04 May 2018 9,033 2 View
I have been searching literature to proceed with a metabolic network which include the detailing of a process including around 38 variables and 25 unknown parameters. How feasible is it to make a...
31 December 2017 1,531 2 View
It is assumed that proteins having a sequence similarity of ~35% tend to fold in a similar pattern. If so, can this be used to predict the binding properties of the two proteins based on their...
01 February 2017 5,438 0 View
Opti-MEM is a modification of MEM only. Why is a low FBS required during transfetion?
05 June 2016 4,732 11 View
I have ordered lipofectamine 2000 from Thermo Fishers Scientific. The product has been provided with a protocol, but does not clearly reports the incubation times. I have tried transfection as per...
05 June 2016 6,705 8 View
I have tried the ligation procedure quite many times, but have failed to get colonies after transformation. I get no colonies after transformation. Also I have tested the competence of competent...
01 February 2016 4,634 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...
03 August 2024 2,366 0 View