Why does the error says GDC server down while I try to download data from the GDC portal through bioconductor tool?

I am trying to download DNA Methylation Data from the TCGA Bioconductor tool. But whenever I give a query I encounter this error: "Error in value[[3L]](cond) : GDC server down, try to use this...

04 May 2018 8,856 2 View

How feasibile is it to simulate an ODE metabolic model with 38 variables?

I have been searching literature to proceed with a metabolic network which include the detailing of a process including around 38 variables and 25 unknown parameters. How feasible is it to make a...

31 December 2017 1,475 2 View

What is the significance of finding a solution in the null space?

When a "m x n" matrix A is Linearly transformed such that A.x=0, then the solution is expected to be present in the null space. What does this means? And is it easier to find a solution in the...

08 September 2016 2,669 0 View

Is there an alternate for Opti-MEM for lipofectamine transfection? Can I use MEM with low supplemented FBS?

Opti-MEM is a modification of MEM only. Why is a low FBS required during transfetion?

05 June 2016 4,676 11 View

Can anyone provide me with a proper transfection protocol into HEK293 cells with Lipofectamine 2000?

I have ordered lipofectamine 2000 from Thermo Fishers Scientific. The product has been provided with a protocol, but does not clearly reports the incubation times. I have tried transfection as per...

05 June 2016 6,585 8 View

Is there a way to check whether Ligation has worked properly before doing transformation?

I have tried the ligation procedure quite many times, but have failed to get colonies after transformation. I get no colonies after transformation. Also I have tested the competence of competent...

01 February 2016 4,567 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View