Why does the ELISA for COVID19 that the UK just bought many of not work?

More Nico Crudele's questions See All

Are open source PCR primers and cellular reagents of value to locating COVID infections on the global map?

A concern with commercial COVID-19 diagnostic test kits is that they tend to be expensive, and may be difficult to access in places far away from the companies that produce them. In order to...

10 December 2020 5,965 2 View

Has anyone made human RNAse P cDNA PCR primers?

I wonder if the positive control primers and probes authorized by the US CDC for amplifying human RNAse P are specific to the RNAse P chromosomal DNA. My thought process is that if primers are...

01 December 2020 2,364 2 View

What are some experiments that can be done with a microbiology lab to help deal with the covid pandemic?

I run a lab that is equipped with basic microbiology and molecular biology equipment - pipettes, centrifuge tubes, positive pressure flow hood, qPCR machine, vortex, centrifuge, spectrophotometer....

30 November 2020 2,517 10 View

What is a simple and affordable method for protein purification from cell lysate?

The proteins need to be enzymatically active, planning to use Taq. Polymerase for PCR reactions. I am looking to create a protein extract that can be used for diagnostic PCR. So the protein has to...

10 May 2020 7,132 12 View

Why do ELISA tests for SARS-CoV-2 target the antibody instead of the antigen?

I'm wondering why existing ELISA tests for SARS-CoV-2 only target the immune system's antibody against the virus instead of the antigen - (S) spike glycoprotein? Then the assay could detect the...

29 March 2020 3,242 9 View

How to test if chart patterns occure systematic or are just random artifacts in a price-time series?

Given a time series with Events (chart patterns), I want to assess whether events (chart patterns) occur systematic or randomly in a time series. My idea is to identify patterns in a given time...

10 March 2020 2,060 3 View

Can I use the GCPL technique on EC-Lab to obtain charge/discharge curves for a redox flow battery and subsequently calculate VE, CE and EE?

With the setup I'm currently using I obtain VE, CE and EE's of > 100 % and I would like to confirm the method as this is a completely new field for me.

09 July 2019 4,687 3 View

Non celiac gluten sensitivity, why is geographically dependent?

Hello, So I meet a case of a person that in Europe has gluten sensitivity but never got positive in the celiac tests. When travelling outside Europe (Asia, Africa, Cuba) can eat breat and other...

19 October 2018 7,487 1 View

UTR region, where it comes from and how can I modify it?

Dear folks, I was wondering about the UTR region. As far as I understand, it is not in the DNA sequence and so is later added in the mRNA ( Am I right?). Who adds it? Is the RNA polymerase? There...

26 July 2018 6,265 3 View

Is there a clear statistical criterion of a floor-effect?

There is an obvious floor-effect in my data: most of the participans achieved the lowest possible score (which is only -.74 SD from the mean score). Is there a statistical criterion of when to...

20 June 2016 8,713 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Maurice Ekpenyong

In situations or periods of desperation like this; the COVID-19 situation, individuals, organizations etc, will look to exploit the situation. What is happening in UK with the test kits is not new but by God, lives are lost when we think to maximize profit. I totally condemn such an atrocity. The UK has to look elsewhere for functional kits if they feel dissatisfied with the last supplier.

Nico Crudele

Yuan-Yeu Yau I have read this in news articles:

https://www.thetimes.co.uk/article/britain-has-millions-of-coronavirus-antibody-tests-but-they-don-t-work-j7kb55g89

according to this article the problem is a lack of sensitivity in the assay:

https://www.bloomberg.com/news/articles/2020-04-07/new-test-hopes-dashed-as-u-k-finds-antibody-kits-don-t-deliver

https://www.economist.com/britain/2020/04/04/whats-gone-wrong-with-covid-19-testing-in-britain