Nico Crudele

8 Questions 16 Answers 0 Followers

Questions related from Nico Crudele

Why are my Cyclic voltammetry results changing between measurements?

I am trying to detect Calcium in PBS. I am use an Ag/AgCl reference electrode, glassy carbon working electrode and platinum wire counter electrode. I am using PBS pH 8.5 with 0.5 MgCl2 as the...

24 May 2022 1,309 8 View

Is carbon paste the best working electrode to use for detection of small biological molecules on a microfluidic device?

I am trying to build an electrochemical sensor for detecting a metabolite produced by yeast, through an engineered pathway. I found a protocol for detecting ethanol using alcohol dehydrogenase, in...

04 February 2022 6,907 0 View

Are open source PCR primers and cellular reagents of value to locating COVID infections on the global map?

A concern with commercial COVID-19 diagnostic test kits is that they tend to be expensive, and may be difficult to access in places far away from the companies that produce them. In order to...

11 December 2020 6,107 2 View

Has anyone made human RNAse P cDNA PCR primers?

I wonder if the positive control primers and probes authorized by the US CDC for amplifying human RNAse P are specific to the RNAse P chromosomal DNA. My thought process is that if primers are...

02 December 2020 2,532 2 View

What are some experiments that can be done with a microbiology lab to help deal with the covid pandemic?

I run a lab that is equipped with basic microbiology and molecular biology equipment - pipettes, centrifuge tubes, positive pressure flow hood, qPCR machine, vortex, centrifuge, spectrophotometer....

01 December 2020 2,689 10 View

What is a simple and affordable method for protein purification from cell lysate?

The proteins need to be enzymatically active, planning to use Taq. Polymerase for PCR reactions. I am looking to create a protein extract that can be used for diagnostic PCR. So the protein has to...

11 May 2020 7,274 12 View

Why does the ELISA for COVID19 that the UK just bought many of not work?

Has anyone analyzed exactly what part of the new ELISA test the UK gov't bought doesn't work? Is the antibody not specific enough?

07 April 2020 7,376 4 View

Why do ELISA tests for SARS-CoV-2 target the antibody instead of the antigen?

I'm wondering why existing ELISA tests for SARS-CoV-2 only target the immune system's antibody against the virus instead of the antigen - (S) spike glycoprotein? Then the assay could detect the...

30 March 2020 3,374 9 View