I ran 96 well plate with temperature gradient 55-65 and a melt curve. It took unreasonably long to run melt curve, after two hours of running melt curve I stopped it from CFX manager software, which did not show any response. Finally I had to power off the cycler. After restarting both computer and the cycler, the run gets stuck at initializing step ('starting run'). If anybody has such experience please share your solution/suggestion?