What is the soft agar which is used in double layer agar to determine the titer of viruses?

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How to find ECC from radiation pattern on cst ?

i need to find ECC for 4-MIMO antenna using radiation pattern on CST

16 July 2024 7,291 1 View

What are the principles of physical chemistry?

what are the main points

10 July 2024 9,236 5 View

Which theories support the relationship between organizational and external variables and d strategy adoption?

hello, which management theories can be used to address antecedents of digital strategy adoption ?

22 June 2024 836 4 View

Can you help me the optimization micro grid using multi-objective?

Optimal sizing microgrid off grid case study ''I choose the case study""so in our research using PV and WT .battery storage and diesel generator .we have the data formation Hourly the solar ir and...

20 June 2024 5,086 0 View

Winprop feko software ?

please any one work on winprop feko software to simulate 5G antenna base station and network coverage send me LTE air interface file (.wst) that needed for network analysis

02 June 2024 1,229 0 View

How to avoid formation of coagulation in core-shell emulsion polymers?

good morning, I'm synthesizing emulsion polymers via core-shell technology, but I face problems during the synthesis as I find large amounts of coagulation in the form of sand-like particles. Why...

20 May 2024 9,905 5 View

Could i know excellent physicochemical, mechanical, and biocompatibility properties, as required for potential bioinks for chronic wound healing.?

could i know excellent physicochemical, mechanical, and biocompatibility properties, as required for potential bioinks for chronic wound healing.

14 May 2024 8,645 1 View

How to perform DNA extraction for Long read sequencing using QIAgen genomic buffer set kit from Aspergillus isolates?

Greetings everyone, I am currently tasked with conducting DNA extraction from Aspergillus isolates, utilizing the QIAgen genomic buffer set kit. Regrettably, not all the components outlined in the...

03 May 2024 4,985 0 View

Is there a open source raman spectroscopy program for pattern identification analog to QualX and Match for XRD patterns?

I have two patterns one is pure Brucite and the second is brucite with my adsorbed material and I want to identify the impurities on the second pattern. Thank you for any help you may provide.

22 April 2024 7,592 0 View

How can I extract location Points of Interest using a Bayesian network?

I want to understand how to configure and apply such a model to identify significant or popular locations in a geographic dataset.

22 April 2024 2,363 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,755 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,563 6 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View