temperature, storage, bacteriophage
It will depend upon the phage that you are using. However if the lysate is sterile then in most cases it will be ok to transport at room temperature although be aware that the titer might go down a bit.
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After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....
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