Anybody can recommend to me a viral DNA/RNA extraction kit from stool samples with good yield?

I want to extract viral DNA/RNA from stool samples for sequencing. Does anybody have experience with the kits and can recommend one?

10 November 2018 3,176 3 View

What is the best and safe method used in your laboratory to inhibit the effectiveness of Ethidium Bromide used in DNA dyeing?

I am looking for different techniques for Ethidium Bromide inhibition after the PCR gel electrophoresis to choose the most easy and safe method to write a SOP for a good laboratory practice.

10 November 2017 5,173 9 View

What is the differences between Methylation-Specific PCR and Sequencing after bisulfite treatment primers and how to design both?

I have a primer for a Methylation-Specific PCR (MSP) taken from the previous study and I want to design a primer for sequencing bisulfite-converted DNA for the same region that MSP primer bind to...

04 May 2017 8,795 7 View

What should be the differences and the range between tm of primers and annealing in RT-PCR?

03 April 2017 562 12 View

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection?

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection? After collection of sample and cut off the swab tip in the UTM then vigorously agitate swabs in medium for...

11 December 2016 7,134 2 View

Possibilities that made my RT-PCR wrong?

10 November 2016 6,073 11 View

I need to know the most recent Artificial Intelligent (AI) and Evolutionary Algorithm (EA) Optimization methods (Published in 2015 and 2016) ?

I need to know the most recent Artificial Intelligent (AI) and Evolutionary Algorithm (EA) Optimization methods to solve Optimal Power Flow Optimization problem.

09 October 2016 6,709 9 View

No title

09 October 2014 2,748 1 View

How to tackle the challenge of having inexperienced users in games when it comes to User-Centred Design for Gamified Services?

UCD is important for the engagement of users with products. However, in developing 'Gamified' services you need users who have the logic of games design and development. Using inexperienced users...

11 December 2013 7,422 5 View

How to tackle the challenge of having inexperienced users in games when it comes to User-Centred Design for Gamified Services?

UCD is important for the engagement of users with products. However, in developing 'Gamified' services you need users who have the logic of games design and development. Using inexperienced users...

04 December 2013 2,356 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View