???
Error message when using map Sanger data analysis "Subtask {Compose alignment} is failed: No read satisfy minimum identity criteria" typically occurs when I am attempting to map or align...
01 September 2023 4,182 1 View
I want to extract viral DNA/RNA from stool samples for sequencing. Does anybody have experience with the kits and can recommend one?
10 November 2018 3,220 3 View
I am looking for different techniques for Ethidium Bromide inhibition after the PCR gel electrophoresis to choose the most easy and safe method to write a SOP for a good laboratory practice.
10 November 2017 5,229 9 View
I am looking for a good program that helps me to calculate the penetration depth of light depending on some parameters? Any suggestions?
13 July 2017 4,790 4 View
If a subject such as soft tissue of human body, was exposed to a certain light, how we can measure the penetration depth of this light in that subject, practically and NOT by calculation?
12 July 2017 1,095 2 View
I have a primer for a Methylation-Specific PCR (MSP) taken from the previous study and I want to design a primer for sequencing bisulfite-converted DNA for the same region that MSP primer bind to...
04 May 2017 8,854 7 View
If Tm of the both primer was 67C, is it 60C perfect as annealing temperature?
03 April 2017 652 12 View
What are the best methods for nasopharyngeal swab processing for viral q-PCR detection? After collection of sample and cut off the swab tip in the UTM then vigorously agitate swabs in medium for...
11 December 2016 7,203 2 View
Hello, I would ask about RT-PCR for 4 different genes including GAPDH for control sample and the ct value gave above 37 cycle up to 40 in different concentrations of primers so what is the...
10 November 2016 6,123 11 View
I need to know the most recent Artificial Intelligent (AI) and Evolutionary Algorithm (EA) Optimization methods to solve Optimal Power Flow Optimization problem.
09 October 2016 6,736 9 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
How far is the distance with question marks on it? There is a river, where you measure at the point out to the right and there is a low screen. The river is green, what is visible is within the...
24 July 2024 4,405 1 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help
17 July 2024 5,151 5 View
