Anybody can recommend to me a viral DNA/RNA extraction kit from stool samples with good yield?

I want to extract viral DNA/RNA from stool samples for sequencing. Does anybody have experience with the kits and can recommend one?

19 November 2018 4,518 3 View

What is the best and safe method used in your laboratory to inhibit the effectiveness of Ethidium Bromide used in DNA dyeing?

I am looking for different techniques for Ethidium Bromide inhibition after the PCR gel electrophoresis to choose the most easy and safe method to write a SOP for a good laboratory practice.

27 November 2017 6,868 8 View

What is the best and safe method used in your laboratory to inhibit the effectiveness of Ethidium Bromide used in DNA dyeing?

I am looking for different techniques for Ethidium Bromide inhibition after the PCR gel electrophoresis to choose the most easy and safe method to write a SOP for a good laboratory practice.

10 November 2017 5,173 9 View

What is the differences between Methylation-Specific PCR and Sequencing after bisulfite treatment primers and how to design both?

I have a primer for a Methylation-Specific PCR (MSP) taken from the previous study and I want to design a primer for sequencing bisulfite-converted DNA for the same region that MSP primer bind to...

04 May 2017 8,795 7 View

What should be the differences and the range between tm of primers and annealing in RT-PCR?

03 April 2017 562 12 View

Why does the anomeric effect is more visible in nonplar solvents?

???

01 February 2017 8,534 0 View

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection?

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection? After collection of sample and cut off the swab tip in the UTM then vigorously agitate swabs in medium for...

11 December 2016 7,134 2 View

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection?

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection? After collection of sample and cut off the swab tip in the UTM then vigorously agitate swabs in medium for...

03 December 2016 6,974 2 View

Possibilities that made my RT-PCR wrong?

10 November 2016 6,073 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View