Anybody can recommend to me a viral DNA/RNA extraction kit from stool samples with good yield?

More Arwa Mujahid Abdullah Al-Shuwaikh's questions See All

Anybody can recommend to me a viral DNA/RNA extraction kit from stool samples with good yield?

I want to extract viral DNA/RNA from stool samples for sequencing. Does anybody have experience with the kits and can recommend one?

19 November 2018 4,685 3 View

What is the best and safe method used in your laboratory to inhibit the effectiveness of Ethidium Bromide used in DNA dyeing?

I am looking for different techniques for Ethidium Bromide inhibition after the PCR gel electrophoresis to choose the most easy and safe method to write a SOP for a good laboratory practice.

27 November 2017 7,026 8 View

What is the best and safe method used in your laboratory to inhibit the effectiveness of Ethidium Bromide used in DNA dyeing?

I am looking for different techniques for Ethidium Bromide inhibition after the PCR gel electrophoresis to choose the most easy and safe method to write a SOP for a good laboratory practice.

10 November 2017 5,229 9 View

What is the differences between Methylation-Specific PCR and Sequencing after bisulfite treatment primers and how to design both?

I have a primer for a Methylation-Specific PCR (MSP) taken from the previous study and I want to design a primer for sequencing bisulfite-converted DNA for the same region that MSP primer bind to...

04 May 2017 8,854 7 View

What should be the differences and the range between tm of primers and annealing in RT-PCR?

If Tm of the both primer was 67C, is it 60C perfect as annealing temperature?

03 April 2017 652 12 View

Why does the anomeric effect is more visible in nonplar solvents?

???

01 February 2017 8,590 0 View

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection?

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection? After collection of sample and cut off the swab tip in the UTM then vigorously agitate swabs in medium for...

11 December 2016 7,203 2 View

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection?

What are the best methods for nasopharyngeal swab processing for viral q-PCR detection? After collection of sample and cut off the swab tip in the UTM then vigorously agitate swabs in medium for...

03 December 2016 7,122 2 View

Possibilities that made my RT-PCR wrong?

Hello, I would ask about RT-PCR for 4 different genes including GAPDH for control sample and the ct value gave above 37 cycle up to 40 in different concentrations of primers so what is the...

10 November 2016 6,123 11 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Anupam Gautam

Dear Arwa,

may be this link can help you

https://mobio.com/us/shop/dna-isolation/di-microbial/allprep-powerviral-dna-rna-kit/

Regards,

Veysel Soydal Ataseven

We have been using the commercial kits, such as Vivantis GF-25 or Qiagen MiniCador. Both kits have a good result.

Arwa Mujahid Abdullah Al-Shuwaikh

@Anupam Gautam @Veysel Soydal Ataseven Thank you for your kind cooperation :)