Why does the knowledge of the past climate change events critical for understanding future climate change?

Relationship between past meteorological records and future predictions of climate change phenomena.

08 October 2017 1,167 14 View

Why is the mathematics skills steadily declining at all levels of our educational institutions?

Why are students performing so poorly in mathematics in schools and in standardized achievement tests?

08 October 2017 3,818 12 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View

HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View