does anyone already have a problem of precipitation with MHBII from sigma (Ref 90922)??
When i autoclave my media it's clear but few days after it appears a white precipitate even if the bottle has not be open.
I put the bottle for 48h at 37°C, nothing grew.
Any idea?
Thanks
There are two reasons for such precipitation usually. One is degradation/structural change of media component during the time of steam sterilization. It can be confirmed by vacuum filtering media inside LAF and check for contamination and precipitations.
Second is the expiry date. Check the let you purchased has passed shelf life period
Did you store it in the dark as instructed by Sigma's data sheet? An alternative is to make the medium from scratch while omitting the salts (prepare salt soln. separately and then add to autoclaved medium after cooling to RT). I suspect that the salts are precipitating.
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