Why does my hydrogel make the pH of the medium acidic?

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Riaz A. Khan

There are enough hydrolysable H generating protons to change pH to acidic, check your preparation molar ratios of reactants?

Steingrimur Stefansson

Hi Mohammad Sadegh Aghajanzadeh . Some gelatins are made by acid hydrolysis of collagens. You can try dissolving the gelatin in buffers that do not interfere with Schiff base formation, PBS, HEPES, etc.

Ahmed Nada

Schiff's base reaction is reversible reaction and you need to go for one more step for reduction the imine bond to single bond. reducing agent such like sodium borohydride