In my experiment, I found that the expression level of mRNA is higher, while the protein level which measured by western blot for the same gene of interest is low abundant!
How can this fact be explained?
We trying to find ways to discover novel gene or to identify the functions of novel gene that we have found. Gene (X) e.g., is gene decoded by eukaryotic translation termination factor 1 (eRF1)....
11 December 2017 9,344 1 View
Finally, we got the microarray results assessment from Agilent Technologies: 2 Color Gene Expression. For the feature description, a hit was defined as a positive fluorescence signal for all spots...
08 September 2017 867 0 View
I am attempting to run my SNP dataset in STRUCTURE, at 10K burnin and 10K MCMC reps. I have 7864 loci and 84 individuals. so I ran for K=1 to K=20, at 5 iterations. Now, I am waiting days on my...
08 September 2017 7,273 0 View
I would like to determine gene relative expression in real-time PCR using the comparative CT method (2-ΔΔCt), using three internal control instead of one gene.
07 August 2017 3,018 7 View
Hello, I'm looking forward to some tools (software) for microarray data analysis which has been measured from the whole organism genes.
06 July 2017 4,264 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View