Why does it happen?

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Could you please provide information about the low grades ores available worldwide?

If an expert kindly give me some detailed information on low grades ores ,where are these ores found and if there is still demand for this type of ore? If yes, what kind of low grade ore wastes...

23 October 2021 9,569 1 View

I get 90% depletion in mRNA level while there is no change in protein level by CRISPR/Cas9. Why does it happen?

I performed CRISPR/Cas9 genome editing on my cells. As a pool, they were mixed and depletion did not seem that good. I decided to do single cell cloning. After that, I checked the depletion on...

24 December 2019 3,148 2 View

How can I test soil Eh (ORP) in the laboratory using platinum ORP probe?

Dear colleagues, I collected soil samples 6 months ago and after I homogenize and grind them I kept them in polyethylene bags. I want to check their Eh value now. However I suspect that long...

22 March 2017 2,454 2 View

Can anyone explain me how to determine actual concentration of As in digested soil sample, considering DF, WF, CF and VF ?

If I use 0.5 g soil + 9 ml HNO3 + 3 ml HCI for microwave digestion. And I take only 5 ml of supertant from this digest and dilute it to 25 ml, by adding 5 ml of 5 % KI and 5 % ascorbic acid for...

09 February 2016 7,902 6 View

Is there anybody who used Claisse Bee (Fusion Method) instead of microwave digestion to prepare solutions from soil and plant samples for AAS?

I want to detect As concentration in soil, plant and hair samples. However, microwave digestion is time consuming and my samples are not digested completely. Instead of microwave digestion, I...

21 January 2016 6,330 4 View

How can I decrease As (V) to As (III) for HV-AAS analysis?

What is the best way to decrease Arsenic (V) to Arsenic (III) for HV-AAS analysis? I am used to use 2 ml % 20 KI and 2 ml HCI (37%) as explained in Schimadzu HVG-1 manual. However potassium iodide...

09 November 2015 1,789 3 View

International research team - UPDATE

This topic is now closed. thanks for your interest.

01 January 1970 4,212 20 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View