Why does glycogen biosynthesis require UDP moiety in glycosyl transferase and not ADP?

More Sansrity Sinha's questions See All

Electroactive surface area of bare and the synthesized material?

For electrochemical sensors, the electroactive surface area (ESA) show higher value for the working material as compared to bare (reported in several papers). My bare electrode is FTO and working...

17 January 2021 7,220 3 View

How can i select suitable gene sequence at Ring stage of Plasmodium Falciparum for primer design?

I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...

15 December 2020 6,954 3 View

How to calculate sample size?

The current study Digital Marketing in Indian and its Impact on Consumer behavior in post and Pre-covid-19 in Agra a district of the state utttar pradesh India has to be taken up . How should I go...

06 December 2020 5,614 4 View

No title

I have tried using PowerSoil Pro kit from Qiagen with slight modifications- incubating at 65 degree for 10 mins after adding solution CD1, increasing the number of washes with CD5 to 3 times and...

27 October 2020 7,782 3 View

Are oil glands present in sandalwood? Or it is a byproduct of sapwood to heartwood formation?

I came to know that there is no oilglands in sandalwood and it is a byproduct during transformation of sapwood to heartwood. Is it true?

04 October 2020 8,216 2 View

From where should I get the free climate data for downloading ?

Hello all, I need a small help for downloading free climate data sets for annual mean temperature, rainfall, flood and drought for period of 30 years of Bihar, India. Please suggest me some...

04 October 2020 1,939 19 View

Need CIF files (XRD) for iron ore sinter phases?

Hi, Can someone share the CIF file (XRD analysis) for SFCA, SFCA I, SFCAII, CF, Ca-Magnetite. Can't find these in COD Thanks, Moni

25 August 2020 7,377 2 View

How to identify the planes and directions in the pole figures obtained from TSL OIM Analysis 7?

I have provided the images reported in journal papers. Which technique in TSL OIM Analysis 7 is used to identify the planes and directions in the EBSD map? How is it colour coded with the pole...

03 August 2020 8,078 1 View

I would like to make my Research papers Private to uploaded in Research Gate. How can I do thins?

Dear Sir/Madam, I want to make Private the loaded research papers in the Research Gate . How can I do this? with regards Sincerely H N Sinha

23 July 2020 4,520 4 View

Can pearlite form (traces/locally) in an ultra-low carbon steel with a carbon percentage of <0.005%?

If the ultra-low carbon steel is held at high isothermal temperatures will it be possible to form pearlite? How can the formation of pearlite be confirmed other than morphological confirmation?

14 July 2020 9,251 4 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

How to immobolise streptavidin to polyacrylamide using EDC/NHS?

Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...

17 December 2020 7,944 2 View

I have proposal to extract HPA from snails, dos any researcher have protocol of HPA (Helix pomata aglutinin) extraction?

Lectin fro Helix pomatia agglutinin used to detect O-Linked glycosylation

21 October 2020 6,139 3 View

How do I Glycosylate a Protein?

I am in my first year of a biology degree and i'm struggling with some coursework. How would i go about setting up an expression system to create a glycoprotein. I understand how to over express a...

19 March 2020 6,605 4 View

Is there any alternative for glycogen solution in DNA precipitation?

Hi I am a master student working on Parrafin-embedded tissue to extract DNA and I could't find glycogen 20mg/ml solution used in the DNA precipitation an I am wondering if there is any...

18 February 2020 1,504 1 View

Can I study three partner interactions with Surface Plasmon Resonance?

We are looking at whether a peptide: RNA as a complex can bind to a glycosylated protein. We have established with SPR that the Peptide binds to RNA. Is it possible to study the binding of the...

01 February 2020 5,807 2 View

Quantifying glycosylated proteins on a SDS-PAGE?

What is the best way to approach (unexpected) glycosylated proteins on a western blot (or in general)? I am looking at some potential anti-fibrotic treatments (Tx) using 2D cultured primary human...

01 February 2020 7,303 3 View

TRIzol RNA extraction: No pellet after isopropanol precipitation even with glycogen?

So I'm using TRIzol for RNA extraction, but suddenly I'm getting no pellet during the isopropanol step *even though I added glycogen.* A few weeks ago I: 1. Took a large quantity of bacteria 2....

05 January 2020 8,370 5 View

TRIzol RNA extraction: No pellet after isopropanol precipitation even with glycogen?

So I'm using TRIzol for RNA extraction, but suddenly I'm getting no pellet during the isopropanol step *even though I added glycogen.* A few weeks ago I: 1. Took a large quantity of bacteria 2....

31 December 2019 3,856 5 View

Adam B Shapiro

I'll offer a hypothesis. By using UDP-sugars as substrates for most glycosyl transferases instead of ADP-sugars, the possibility of cross-talk or interference between metabolic enzymes is reduced. ADP sugars might act as competitive inhibitors of some of the many enzymes that use adenosine nucleotides as substrates.

Dyoni M. Oliveira

I agree with Shapiro and I add that UDP-dependent enzymes are very conserved enzymes.

Sansrity Sinha

thanx for your answer.